A genetically encoded tool to increase cellular NADH/NAD(+) ratio in living cells.
Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha N, Yao C, Goodman RP, Cross JR, Cracan V
Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218628 | pLVX-TetOne-Puro-EcSTH-FLAG | This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells. |
| 218629 | pLVX-TetOne-Puro-mitoEcSTH-FLAG | This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells. |
| 218630 | pLVX-TetOne-Puro-LUC | This plasmid contains luciferase under doxycycline inducible control. |
| 218631 | pLVX-TetOne-Puro-LbNOX-FLAG | This plasmid contains LbNOX under doxycycline inducible control. |