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A genetically encoded tool to increase cellular NADH/NAD(+) ratio in living cells.

Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha N, Yao C, Goodman RP, Cross JR, Cracan V
Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218628pLVX-TetOne-Puro-EcSTH-FLAGThis plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
218629pLVX-TetOne-Puro-mitoEcSTH-FLAGThis plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
218630pLVX-TetOne-Puro-LUCThis plasmid contains luciferase under doxycycline inducible control.
218631pLVX-TetOne-Puro-LbNOX-FLAGThis plasmid contains LbNOX under doxycycline inducible control.
218667pLVX-TetOne-Puro-mitoLbNOX-FLAGpLVX-TetOne-Puro-mitoLbNOX-FLAG

Antibodies from Article