John Dueber Lab: Enhanced peroxisome capacity enablescompartmentalization of a multi-enzyme pathway
Dueber Lab
Unpublished
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218587 | pKS1461 | Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP |
| 218588 | pKS1469 | Express peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate |
| 218589 | pJJB1439 | Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol |
| 218590 | pJJB1559 | Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer |
| 218591 | pJJB170 | Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c |
| 218592 | pJJB1338 | Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22 |
| 218593 | pJJB1340 | Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11 |
| 218594 | pSKT001 | Express degron assay: Degradation tag-YFP-ePTS1 |
| 218595 | pJJB1357 | Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1 |
| 218596 | pJJB207 | Express degron assay: Degradation tag-YFP-ePTS1 |
| 218597 | pJJB711 | Express Pex11 |
| 218598 | pJJB714 | Express Pex34 |
| 218599 | pJJB715 | Express Pex25 |
| 218600 | pJJB716 | Express Pex27 |
| 218601 | pJJB717 | Express Pex15 |
| 218602 | pJJB718 | Express Pex3 |
| 218603 | pJJB719 | Express Pex19 |
| 218604 | pJJB1332 | Express Pex5 |
| 218605 | pJJB1333 | Express Pex17 |
| 218606 | pJJB1334 | Express Pex14 |
| 218607 | pJJB1335 | Express Pex28 |
| 218608 | pJJB1336 | Express Pex32 |
| 218609 | pJJB1502 | Express Pex29 |
| 218610 | pJJB1503 | Express Pex30 |
| 218611 | pJJB1504 | Express Pex31 |
| 218612 | pJJB1505 | Express Pex35 |
| 218613 | pJJB1535 | Express Pex5 |
| 218615 | pJJB1401 | Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12 |
| 218616 | pJJB1402 | Express Pex15, Pex22, Pex4, Pex1, and Pex6 |
| 218617 | pJJB1536 | Express Pex5 and Pex11 |
| 218618 | pJJB1376 | Express Pex5, Pex3, Pex19, and Pex8 |
| 218619 | pJJB1577 | Express Pex5, Pex2, Pex17, and Pex10 |
| 218620 | pJJB1578 | Express Pex22, Pex6, Pex15, and Pex8 |
| 218622 | pJJB1426 | Express tNCS to the peroxisome for toxicity assay |
| 218623 | pJJB1462 | Express mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV protease |
| 218624 | pJJB1463 | Express mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV protease |
| 218625 | pJJB1465 | Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c |
| 218626 | pJJB1561 | Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer |
| 218627 | pJJB1734 | Express amorphadiene synthase (ADS) |