Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals.
Kenny TC, Khan A, Son Y, Yue L, Heissel S, Sharma A, Pasolli HA, Liu Y, Gamazon ER, Alwaseem H, Hite RK, Birsoy K
Cell Metab. 2023 Jun 6;35(6):1057-1071.e12. doi: 10.1016/j.cmet.2023.04.003. Epub 2023 Apr 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218521 | plentiCRISPR-v1 GFP FLVCR1_sg5 | sgRNA targeting human FLVCR1 |
| 218522 | plentiCRISPR-v2 Puro FLVCR1_sg5 | sgRNA targeting human FLVCR1 |
| 218523 | plentiCRISPR-v2 Puro CHKA_sg2 | sgRNA targeting human CHKA |
| 218524 | plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1 | sgRNA targeting human KIAA0141/DELE1 |
| 218525 | plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2 | sgRNA targeting human KIAA0141/DELE1 |
| 218527 | plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA | sgRNA targeting human EIF2AK2/PKR |
| 218528 | plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA | sgRNA targeting human EIF2AK4/GCN2 |
| 218529 | plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA | sgRNA targeting human EIF2AK1/HRI |
| 218530 | plentiCRISPR-v2 Puro SPNS1_sg1 | sgRNA targeting human SPNS1 |
| 218531 | plentiCRISPR-v2 Puro SPNS1_sg2 | sgRNA targeting human SPNS1 |
| 218533 | pMXS-IRES-Blast FLVCR1/FLVCR1a | human FLVCR1/FLVCR1a cDNA |
| 218534 | pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG | human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA |
| 218535 | pMXS-IRES-Blast FLVCR1b | human FLVCR1b cDNA |
| 218536 | pMXS-IRES-Blast FLVCR1b-3X FLAG | human FLVCR1b with C-terminal 3X_FLAG cDNA |
| 218537 | pMXS-IRES-Blast FLVCR2 | human FLVCR2 cDNA |
| 218538 | pMXS-IRES-Blast SLC44A1 | human SLC44A1 cDNA |
| 218539 | pMXS-IRES-Blast SLC5A7 | human SLC5A7 cDNA |
| 218540 | pMXS-IRES-Blast CHKA | human CHKA cDNA |
| 218541 | pMXS-IRES-Blast CG1358 | Drosophila CG1358 cDNA (human codon optimized) |
| 218666 | plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA | sgRNA targeting human EIF2AK3/PERK |