Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes.
Lautenschlager N, Schmidt K, Schiffer C, Wulff TF, Hahnke K, Finstermeier K, Mansour M, Elsholz AKW, Charpentier E
Front Bioeng Biotechnol. 2024 Jun 7;12:1395659. doi: 10.3389/fbioe.2024.1395659. eCollection 2024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218505 | pEC3079 (p7INT-Pveg_lacI) | integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase |
| 218508 | pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant)) | integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase |
| 218509 | pEC3115 (pSpy0K6-P23_mKate2~*ssrA) | integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination |
| 218511 | pEC2861 (pSpy1C-PgyrA_ffluc) | replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter |
| 218512 | pEC3105 (pSpy1C-PxylS2_ffluc) | replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter |
| 218513 | pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc) | replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase |
| 218514 | pEC3109 (pSpy1C-Plac(1)_ffluc) | replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor |
| 218515 | pEC3006 (pSpy1C-PZn_ffluc) | replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes |
| 218778 | pEC3111 (pSpy1C-Plac(3)_ffluc) | replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor |
| 220248 | pEC2483 (pSpy1C) | replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression |
| 220279 | pEC2735 (pSpy0C2) | integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination |
| 220280 | pEC2796 (pSpy0C4) | integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination |
| 220306 | pEC3135 (pERASE) | Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS* |