Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes.
Lautenschlager N, Schmidt K, Schiffer C, Wulff TF, Hahnke K, Finstermeier K, Mansour M, Elsholz AKW, Charpentier E
Front Bioeng Biotechnol. 2024 Jun 7;12:1395659. doi: 10.3389/fbioe.2024.1395659. eCollection 2024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218505 | pEC3079 (p7INT-Pveg_lacI) | integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase |
| 218508 | pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant)) | integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase |
| 218509 | pEC3115 (pSpy0K6-P23_mKate2~*ssrA) | integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination |
| 220306 | pEC3135 (pERASE) | Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS* |