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Oded Regev Lab: RNA molecules display distinctive organization at nuclear speckles

Unpublished

Plasmids from Article

ID Plasmid Purpose
218392WT_S1-H13-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
218393WT_S7-H13-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
218394MUT_S1-H13-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
218395MUT_S1-H1,NR3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
218396MUT_S7-H13-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
218397MUT_N-H13-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
218398MUT_S1-N3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site
218399MUT_S1-spacer-H13-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
218400WT_H1-S13-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intron

Antibodies from Article