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A series of vectors for inducible gene expression in multidrug-resistant Acinetobacter baumannii.

Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 10.1128/aem.00474-24. Epub 2024 Aug 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218357pMApra-PtacEmpty vector with lacIq-Ptac expression system
218358pMApra-Plac-SOEmpty vector with lacIq-Plac expression system
218359pMApra-PtolEmpty vector with XylS-Pm expression system
218360pMApra-Ptet1Empty backbone with tetR-PtetA expression system with single tetR operator site
218361pMApra-Ptet2Empty backbone with tetR-PtetA expression system with two tetR operator sites
218363pUC18T-mini-Tn7T-hph-ParaEmpty backbone for Tn7-site insertion with araC-ParaBAD expression system
218364pUC18T-mini-Tn7T-hph-PtacEmpty backbone for Tn7-site insertion with lacIq-Ptac expression system
218365pUC18T-mini-Tn7T-hph-Ptac-VSVGEmpty backbone for Tn7-site insertion with lacIq-Ptac expression system and C-terminal VSVG fusions
218366pUC18T-mini-Tn7T-hphEmpty backbone for Tn7-site insertion
218367pUC18T-mini-Tn7T-hph-Ptet1Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site
218368pUC18T-mini-Tn7T-hph-Ptet2Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites
218369pUC18T-mini-Tn7T-hph-PtolEmpty backbone for Tn7-site insertion with XylS-Pm expression system
218370pUC18T-mini-Tn7T-hph-lacZEmpty backbone for lacZ fusions
218371pUC18T-mini-Tn7T-hph-dCas9Expression of dCas9 for CRISPR-interference
218372pWH1266-Apra-sgRNAsgRNA expression plasmid for CRISPR-intereference

Antibodies from Article