dCas13-mediated translational repression for accurate gene silencing in mammalian cells.
Apostolopoulos A, Kawamoto N, Chow SYA, Tsuiji H, Ikeuchi Y, Shichino Y, Iwasaki S
Nat Commun. 2024 Mar 11;15(1):2205. doi: 10.1038/s41467-024-46412-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233608 | pCAGEN-PspCas13b-NES-mCherry | Expression of PspCas13b in mammalian cells |
| 233609 | pCAGEN-dPspCas13b-NES-mCherry | Expression of catalytically-inactive PspCas13b in mammalian cells |
| 233610 | pCAGEN-dPspCas13b-4EHP-NES-mCherry | Expression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cells |
| 233613 | pC016-gPsp-EGFP_start_codon | Expression of guide RNA targeting the start codon of the EGFP gene for PspCas13b |