Evolving membrane-associated accessory protein variants for improved adeno-associated virus production.

Schieferecke AJ, Lee H, Chen A, Kilaru V, Krish Williams J, Schaffer DV
Mol Ther. 2024 Feb 7;32(2):340-351. doi: 10.1016/j.ymthe.2023.12.015. Epub 2023 Dec 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
218251	pCW57-CMV-MAAP-SL08V9	1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
218250	pCW57-CMV-MAAP-SL01V9	1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
218249	pCW57-CMV-MAAP-SL01V6	1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
218248	pCW57-CMV-MAAP-SL01	1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
218247	pCW57-CMV-MAAP-SL08	1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
218246	pCW57-CMV-MAAP9	1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.
218245	pCW57-CMV-MAAP6	1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.
218244	pCW57-CMV-MAAP2	1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.