Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements.
Chardon FM, McDiarmid TA, Page NF, Martin B, Domcke S, Regalado SG, Lalanne JAE, Calderon D, Starita LM, Sanders SJ, Ahituv N, Shendure J
bioRxiv [Preprint]. 2023 Mar 28:2023.03.28.534017. doi: 10.1101/2023.03.28.534017. PubMed Article
Chardon FM, McDiarmid TA, Page NF, Martin B, Domcke S, Regalado SG, Lalanne JAE, Calderon D, Starita LM, Sanders SJ, Ahituv N, Shendure J
bioRxiv [Preprint]. 2023 Mar 28:2023.03.28.534017. doi: 10.1101/2023.03.28.534017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218234 | piggyFlex | A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers. |