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Addgene

Multiplex, single-cell CRISPRa screening for cell type specific regulatory elements.

Chardon FM, McDiarmid TA, Page NF, Martin B, Domcke S, Regalado SG, Lalanne JAE, Calderon D, Starita LM, Sanders SJ, Ahituv N, Shendure J
bioRxiv [Preprint]. 2023 Mar 28:2023.03.28.534017. doi: 10.1101/2023.03.28.534017. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218234piggyFlexA piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

Antibodies from Article