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The FXR1 network acts as signaling scaffold for actomyosin remodeling.

Chen X, Fansler MM, Janjoš U, Ule J, Mayr C
Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225446pcDNA3puro-mGFPExpress mEGFP-fusion protein of interest
225448pcDNA3puro-mGFP-FXR1a-sh5ResistantExpress mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation
225449pcDNA3puro-EGFP-FXR1-isoform bExpress EGFP-fusion protein of FXR1 isoform b
225451pcDNA3puro-EGFP-FXR1-C (379-621)Express mEGFP-fusion protein of FXR1 isoform a (FXR1 fragment (379-621))
225453pcDNA3puro-mGFP-FXR1-N1-KH1mut (T236D H237D)Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D)
225454pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)
225456pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)Express mEGFP-fusion protein of FXR1 isoform a (V361P)
225457pcDNA3puro-mGFP-FXR1a-Ccmut (N202P-V361P)Express mEGFP-fusion protein of FXR1 isoform a (N202P V361P)
225458pcDNA3puro-mGFP-FXR1a-CC1-CC1Express EGFP-fusion protein of FXR1 isoform a (CC1-CC1)
225459pcDNA3puro-mGFP-FXR1a-CC2-CC2Express mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)
225460pcDNA3puro-mGFP-FXR1a-CcswapExpress EGFP-fusion protein of FXR1 isoform a (CCswap)
225461pcDNA3puro-mGFP-FXR1a-I304NExpress mEGFP-fusion protein of FXR1 isoform a (I304N)
225462pcDNA3puro-mGFP-FMR1 isoform1-sh1ResistantExpress mEGFP-fusion protein of FMR1 isoform 1
225463pcDNA3puro-mGFP-FMR1iso1-I304NExpress mEGFP-fusion protein of FMR1 isoform 1 (I304N)
225466pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)Express mEGFP-fusion protein of FMR1 isoform 1 (V427P)
225467pcDNA3puro-mGFP-FXR1a-G266EExpress mEGFP-fusion protein of FXR1 isoform a (G266E)
225469pcDNA3puro-mGFP-TDRD3Express mEGFP-fusion protein of TDRD3
225470pcDNA3puro-mGFP-TOP3BExpress mEGFP-fusion protein of TOP3B
225471pcDNA3puro-mGFP-ROCK2-C (941-1388)Express mEGFP-fusion protein of ROCK2 fragment (941-1388)
225472pcDNA3puro-mGFP-ROCK2Express mEGFP-fusion protein of ROCK2
225473pcDNA3puro-mGFP-ROCK2-N (1-941)Express mEGFP-fusion protein of ROCK2 fragment (1-941)
225475pcDNA3puro-EGFP-GAPDHExpress mEGFP-fusion protein of GAPDH
225476pcDNA3puro-EGFP-GAPDH-CCExpress mEGFP-GAPDH-CC fusion
225477pcDNA3puro-EGFP-GAPDH-RGGExpress mEGFP-GAPDH-RGG fusion
225478pcDNA3puro-EGFP-GAPDH-Tudor-R richExpress mEGFP-GAPDH-Tudor-R fusion
225479pcDNA3puro-EGFP-GAPDH-TudorExpress mEGFP-GAPDH-Tudor fusion
225480pcDNA3puro-EGFP-GAPDH-R richExpress mEGFP-GAPDH-Rrich fusion
225481BPK1520-FXR1-N202S-gRNAExpress gRNA for FXR1
225482pU6-tevopreq1-FXR1-G266EExpress epegRNA for FXR1
225483LsgRNA-FXR1-KH1-gRNAExpress gRNA for FXR1

Antibodies from Article