Auxin-inducible degron 2 system deciphers functions of CTCF domains in transcriptional regulation.

Hyle J, Djekidel MN, Williams J, Wright S, Shao Y, Xu B, Li C
Genome Biol. 2023 Jan 26;24(1):14. doi: 10.1186/s13059-022-02843-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
232800	pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP	Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.
232801	TRE3G-CTCF-WT-HA-MND-zeo	Inducible over expression of wild type version of CTCF.
232802	TRE3G-CTCF-dRBR-HA-MND-zeo	Inducible over expression of RNA-binding domain deficient form of CTCF.