Reduction of oligomer size modulates the competition between cluster formation and phase separation of the tumor suppressor SPOP.
Sabri N, Cuneo MJ, Marzahn MR, Lee J, Bouchard JJ, Gullulu O, Vaithiyalingam S, Borgia MB, Schmit J, Mittag T
J Biol Chem. 2023 Dec;299(12):105427. doi: 10.1016/j.jbc.2023.105427. Epub 2023 Nov 4.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
197005 | pCDNA4/TO-SPOP-myc (R221C) | Mammalian inducible expression vector for myc-tagged SPOP |
197006 | pCDNA4/TO-SPOP-myc (R354H) | Mammalian inducible expression vector for myc-tagged SPOP |
197007 | pCDNA4/TO-SPOP-myc (R121Q) | Mammalian inducible expression vector for myc-tagged SPOP |
197008 | pCDNA4/TO-GFP-SPOP (WT) | Mammalian inducible expression vector for GFP-tagged SPOP |
197009 | pCDNA4/TO-GFP-SPOP (R221C) | Mammalian inducible expression vector for GFP-tagged SPOP |
197010 | pCDNA4/TO-GFP-SPOP (R354H) | Mammalian inducible expression vector for GFP-tagged SPOP |
197011 | His SUMO TEV R354H SPOP 28-359 | E. coli expression vector |
197021 | pCDNA4/TO-SPOP-myc (BACK) | Mammalian inducible expression vector for myc-tagged SPOP |
197022 | pCDNA.3-V5-SPOP (R221C) | Mammalian expression vecor for V5-tagged SPOP |
197023 | pCDNA.3-V5-SPOP (R354H) | Mammalian expression vecor for V5 tagged SPOP |