PINE-TREE enables highly efficient genetic modification of human cell lines.
Frisch C, Kostes WW, Galyon B, Whitman B, Tekel SJ, Standage-Beier K, Srinivasan G, Wang X, Brafman DA
Mol Ther Nucleic Acids. 2023 Jul 17;33:483-492. doi: 10.1016/j.omtn.2023.07.007. eCollection 2023 Sep 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232727 | PE-pegRNA(BG)-nsgRNA(BG) | A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)). |