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Lysosomal TBK1 Responds to Amino Acid Availability to Relieve Rab7-Dependent mTORC1 Inhibition.

Talaia G, Bentley-DeSousa A, Ferguson SM
bioRxiv [Preprint]. 2023 Dec 17:2023.12.16.571979. doi: 10.1101/2023.12.16.571979. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221552pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells
221553pPB-EF1A-Puro-hTBK1-EGFPExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.
221554pPB-EF1A-Puro-hTBK1-E696K-EGFPExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.
221555pPB-EF1A-Puro-mCherry-RAB7Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.
221556pPB-EF1A-Puro-mCherry-RAB7-S72AExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Antibodies from Article