The H3.3 K36M oncohistone disrupts the establishment of epigenetic memory through loss of DNA methylation.
Sinha J, Nickels JF, Thurm AR, Ludwig CH, Archibald BN, Hinks MM, Wan J, Fang D, Bintu L
bioRxiv [Preprint]. 2023 Oct 14:2023.10.13.562147. doi: 10.1101/2023.10.13.562147.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 225690 | pLenti-rTetR(SE)-KRAB-2A-mTurquoise | Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS |
| 225691 | pLenti-rTetR(SE)-HP1a-2A-mTurquoise | Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS |
| 225692 | pLenti-rTetR(SE)-EED-2A-mTurquoise | Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS |
| 225693 | pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise | Lentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLS |
| 225695 | pLenti-rTetR(SE)-VP64-2A-mCherryBSD | Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR |
| 225697 | pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD | Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR |
| 225698 | pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD | Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR |
| 225699 | pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD | Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR |
| 225700 | pLenti-MCS-3AID-HA-2A-mCherryBSD | Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR |
| 225701 | pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD | Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR |
| 225702 | pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD | Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR |
| 225703 | pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD | Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR |
| 225704 | pLenti-MCS-3AID-HA-2A-mTurquoiseBSD | Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR |
| 225872 | pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD | Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR |