An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells.
Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA
bioRxiv [Preprint]. 2023 Dec 12:2023.11.22.568319. doi: 10.1101/2023.11.22.568319.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
220594 | pAAV-EF1a-ArgiNLS-EGFP | Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein. |
220595 | pAAV-EF1a-ArgiNLS-AausFP1 | Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
220596 | pAAV-EF1a-ArgiNLS-mGreenLantern | Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein. |
220597 | pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME) | Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein. |
220599 | pAAV-EF1a-ArgiNLS-mKate2 | Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein. |
220600 | pAAV-EF1a-ArgiNLS-miRFP670 | Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein. |
220601 | pAAV-CAG-FLEX-ArgiNLS-EGFP | Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein |
220602 | pAAV-CAG-FLEX-ArgiNLS-AausFP1 | Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
220605 | pAAV-CAG-fDIO-ArgiNLS-AausFP1 | Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
220606 | pAAV-CAG-fDIO-ArgiNLS-oScarlet | Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein. |
220607 | pAAV-CAG-fDIO-ArgiNLS-mKate2 | Cre-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein. |
220608 | pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet | Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter. |
220610 | pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1 | Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter |
220611 | pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq) | Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor. |
220612 | pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1 | Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter. |
220614 | pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet | Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter. |
220799 | pDG1-PHP.eB | AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes |