An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single cells.
Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA
Proc Natl Acad Sci U S A. 2024 Aug 6;121(32):e2320250121. doi: 10.1073/pnas.2320250121. Epub 2024 Jul 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220594 | pAAV-EF1a-ArgiNLS-EGFP | Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein. |
| 220595 | pAAV-EF1a-ArgiNLS-AausFP1 | Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
| 220596 | pAAV-EF1a-ArgiNLS-mGreenLantern | Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein. |
| 220597 | pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME) | Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein. |
| 220599 | pAAV-EF1a-ArgiNLS-mKate2 | Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein. |
| 220600 | pAAV-EF1a-ArgiNLS-miRFP670 | Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein. |
| 220601 | pAAV-CAG-FLEX-ArgiNLS-EGFP | Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein |
| 220602 | pAAV-CAG-FLEX-ArgiNLS-AausFP1 | Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
| 220605 | pAAV-CAG-fDIO-ArgiNLS-AausFP1 | Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein. |
| 220606 | pAAV-CAG-fDIO-ArgiNLS-oScarlet | Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein. |
| 220607 | pAAV-CAG-fDIO-ArgiNLS-mKate2 | Flp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein. |
| 220608 | pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet | Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter. |
| 220609 | pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1 | Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter. |
| 220610 | pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1 | Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter |
| 220611 | pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq) | Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor. |
| 220612 | pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1 | Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter. |
| 220613 | pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1 | Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter. |
| 220614 | pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet | Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter. |
| 220799 | pDG1-PHP.eB | AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes |