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An arginine-rich nuclear localization signal (ArgiNLS) strategy for streamlined image segmentation of single-cells.

Szelenyi ER, Navarrete JS, Murry AD, Zhang Y, Girven KS, Kuo L, Cline MM, Bernstein MX, Burdyniuk M, Bowler B, Goodwin NL, Juarez B, Zweifel LS, Golden SA
bioRxiv [Preprint]. 2023 Dec 12:2023.11.22.568319. doi: 10.1101/2023.11.22.568319. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220594pAAV-EF1a-ArgiNLS-EGFP Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.
220595pAAV-EF1a-ArgiNLS-AausFP1Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.
220596pAAV-EF1a-ArgiNLS-mGreenLanternConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.
220597pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.
220599pAAV-EF1a-ArgiNLS-mKate2Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.
220600pAAV-EF1a-ArgiNLS-miRFP670 Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.
220601pAAV-CAG-FLEX-ArgiNLS-EGFPCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein
220602pAAV-CAG-FLEX-ArgiNLS-AausFP1 Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.
220605pAAV-CAG-fDIO-ArgiNLS-AausFP1 Flp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.
220606pAAV-CAG-fDIO-ArgiNLS-oScarlet Flp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.
220607pAAV-CAG-fDIO-ArgiNLS-mKate2Cre-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.
220608pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarletNeuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
220610pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
220611pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
220612pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
220614pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarletCre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
220799pDG1-PHP.eBAAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Antibodies from Article