A metabolomics pipeline highlights microbial metabolism in bloodstream infections.
Mayers JR, Varon J, Zhou RR, Daniel-Ivad M, Beaulieu C, Bholse A, Glasser NR, Lichtenauer FM, Ng J, Vera MP, Huttenhower C, Perrella MA, Clish CB, Zhao SD, Baron RM, Balskus EP
Cell. 2024 Jun 11:S0092-8674(24)00579-8. doi: 10.1016/j.cell.2024.05.035.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220473 | pTrcHis_VectorA_short_speG_genomic | heterologous expression under endogenous speG E. coli promoter |
| 220474 | pTrcHis_VectorA_short_speGpromoter_PA4114 | heterologous expression of PA4114 under endogenous speG E. coli promoter |
| 220475 | pTrcHis_VectorA_short_speGpromoter_PA1472 | heterologous expression of PA1472 under endogenous speG E. coli promoter |
| 220476 | pTrcHis_VectorA_short_speGpromoter_PA1377 | heterologous expression of PA1377 under endogenous speG E. coli promoter |
| 220477 | pTrcHis_VectorA_short_speGpromoter_SAT1 (human) | heterologous expression of SSAT1 under endogenous speG E. coli promoter |
| 220478 | pET28a_speG_Nterm_His | heterologous expression for protein purification |
| 220479 | pET28a_PA4114_Nterm_His | heterologous expression of PA4114 for protein purification |
| 220480 | pET28a_PA1472_Nterm_His | heterologous expression of PA1472 for protein purification |
| 220481 | pET28a_PA1377_Nterm_His | heterologous expression of PA1377 for protein purification |
| 220482 | pET28_Human_SAT1_Nterm_His | heterologous expression of SSAT1 for protein purification |
| 220483 | pET28a_speG_Cterm_His | heterologous expression for protein purification |
| 220484 | pET28a_PA4114_Cterm_His | heterologous expression of PA4114 for protein purification |
| 220485 | pET28a_PA1472_Cterm_His | heterologous expression of PA1472 for protein purification |
| 220487 | pET28_Human_SAT1_Cterm_His | heterologous expression of SSAT1 for protein purification |
| 220488 | pgRNA-bacteria_speG_M15 | inducible CRISPRi |
| 220489 | pgRNA-bacteria_speG_E23 | inducible CRISPRi |