Broad range plastid genome editing with monomeric TALE-linked cytosine and dual base editors.
Wang X, Fang T, Lu J, Tripathi L, Qi Y
Plant Biotechnol J. 2024 May 6. doi: 10.1111/pbi.14358.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218171 | pYPQ121-Split-TALED | Express plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA protein |
| 218172 | pYPQ121-mTCBE | Express plastid monomeric TALE-linked CBE, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a human APOBEC3A variant (hA3A-Y130F), and UGI |
| 218173 | pYPQ121-mTCABE-T | Express plastid monomeric TALE-linked CABE-T, composed of TALE, a catalytically impaired, full length DddA variant (E1347A), a TadA-derived deaminase, and UGI |
| 218174 | PYPQ121-DdCBE | Express plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIs |