Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces.
Wang J, Wang K, Deng Z, Zhong Z, Sun G, Mei Q, Zhou F, Deng Z, Sun Y
Nat Commun. 2024 Jul 7;15(1):5687. doi: 10.1038/s41467-024-49987-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218156 | pSCBE3-HF1 | This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces. |
| 218157 | pSCBE3-Hypa | This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces. |
| 218158 | pSCBE3-HF1-Hypa | This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces. |
| 218159 | pSCBE3-NG | This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218160 | pSCBE3-NG-HF1 | This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218161 | pSCBE3-NG-Hypa | This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218162 | pSCBE3-NG-HF1-Hypa | This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218163 | peSCBE3-NG | This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218164 | peSCBE3-NG-HF1 | This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces. |
| 218165 | peSCBE3-NG-Hypa | This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces |
| 218166 | peSCBE3-NG-HF1-Hypa | This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces. |