Modular and integrative activity reporters enhance biochemical studies in the yeast ER.
Martinusen SG, Slaton EW, Nelson SE, Pulgar MA, Besu JT, Simas CF, Denard CA
Protein Eng Des Sel. 2024 Jan 29;37:gzae008. doi: 10.1093/protein/gzae008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218113 | MET-HYGRO | Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out. |
| 218114 | LYS-Leu2 | Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out. |
| 218115 | pY2 | Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter. |
| 218116 | pY3 | Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter. |
| 218117 | pY2-MET-IV | MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus. |
| 218118 | pY2-LYS-IV | LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus. |
| 218119 | pY3-LYS-IV | LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus. |