Continuous evolution of compact protein degradation tags regulated by selective molecular glues.
Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, Deb A, Choudhary A, Fischer ES, Liu DR
Science. 2024 Mar 15;383(6688):eadk4422. doi: 10.1126/science.adk4422. Epub 2024 Mar 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218121 | pJM154 | Hybrid circuit displaying FKBP12 bait and driving expression of luxAB |
| 218123 | pJM166B | For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB |
| 218124 | pJM194A | Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB |
| 218125 | pJM195A | Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII |
| 218126 | pJM185A | Hybrid circuit displaying hsCRBN bait and driving expression of luxAB |
| 218127 | pJM186A | Hybrid circuit displaying hsCRBN bait and driving expression of gIII |
| 218128 | pSD025A | Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg |
| 218129 | pSD139A | Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB |
| 218130 | pSD138A | Hybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxAB |
| 218131 | pSD094B | Hybrid circuit displaying mmCRBN bait and driving expression of gIII |
| 218132 | pSD090A | Hybrid circuit displaying mmCRBN bait and driving expression of luxAB |
| 218133 | pSD113A | For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56 |
| 218134 | pSD103D | For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55 |
| 218135 | pJM189A | For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0. |
| 218136 | pSD057C | For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36. |
| 218137 | pSD068F | Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control. |
| 218138 | pSD080A | Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control. |
| 218139 | pSD141B | Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control. |
| 218140 | pSD118A | Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli |
| 218141 | pSD117A | Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli |