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Evidence for the nuclear import of histones H3.1 and H4 as monomers.

Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ
EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub 2018 Sep 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217962pCL1032Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.
217963pCL1271Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

Antibodies from Article