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A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae.

Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, Moroka P, Schroda M, Deponte M
Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. eCollection 2024. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217994pMBS-838pLEXSY_I-blecherry3_dom_lacZ Destination Vector
217995pMBS-839sAP1 protein secretion signal
217996pMBS-8403xHA epitope tag for protein detection
217997pMBS-843smtHSP60 for protein targeting to mitochondrial matrix
217998pMBS-844Nucleoplasmin NLS for protein targeting to nucleus
217999pMBS-1125Start-methionine
218000pMBS-845Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage
218001pMBS-8463xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage
218002pMBS-847Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage
218003pMBS-848GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage
218004pMBS-8498xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage
218005pMBS-850Receptor binding domain of SARS-CoV-2
218006pMBS-851Spacer to bridge position B3
218007pMBS-857Receptor binding domain of SARS-CoV-2
218008pMBS-858mVenus fluorescent protein
218009pMBS-859mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II
218010pMBS-852Receptor binding domain of SARS-CoV-2
218011pMBS-853mCerulean fluorescent protein
218012pMBS-854mVenus fluorescent protein
218013pMBS-855mCherry fluorescent protein
218014pMBS-856sfGFP fluorescent protein
218015pMBS-872NanoLuc luminescence reporter
218016pMBS-860mVenus fluorescent protein
218017pMBS-861mCerulean fluorescent protein
218018pMBS-864GST-tag fused to TEV Protease cleavage site for protein purification and cleavage
218019pMBS-8718xHis-1xHA-tag for protein detection and purification
218020pMBS-10228xHis-SP20-3xHA-tag for protein detection and purification
218021pMBS-869ER retention signal (DGDL) for protein targeting to ER
218199pCM0-101Multi-Stop for adding 3 stops for each frame
218200pCM0-109SV40 NLS for protein targeting to nucleus
218201pCM0-099Strep-8xHis-tag for protein detection and purification
218202pCM0-1003xHA epitope tag for protein detection
218203pMBS-7231xHA-8xHis-tag for protein detection and purification
218204pCM0-054SV40 NLS for protein targeting to nucleus

Antibodies from Article