A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae.
Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, Moroka P, Schroda M, Deponte M
Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. eCollection 2024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217994 | pMBS-838 | pLEXSY_I-blecherry3_dom_lacZ Destination Vector |
| 217995 | pMBS-839 | sAP1 protein secretion signal |
| 217996 | pMBS-840 | 3xHA epitope tag for protein detection |
| 217997 | pMBS-843 | smtHSP60 for protein targeting to mitochondrial matrix |
| 217998 | pMBS-844 | Nucleoplasmin NLS for protein targeting to nucleus |
| 217999 | pMBS-1125 | Start-methionine |
| 218000 | pMBS-845 | Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage |
| 218001 | pMBS-846 | 3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage |
| 218002 | pMBS-847 | Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage |
| 218003 | pMBS-848 | GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage |
| 218004 | pMBS-849 | 8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage |
| 218005 | pMBS-850 | Receptor binding domain of SARS-CoV-2 |
| 218006 | pMBS-851 | Spacer to bridge position B3 |
| 218007 | pMBS-857 | Receptor binding domain of SARS-CoV-2 |
| 218008 | pMBS-858 | mVenus fluorescent protein |
| 218009 | pMBS-859 | mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II |
| 218010 | pMBS-852 | Receptor binding domain of SARS-CoV-2 |
| 218011 | pMBS-853 | mCerulean fluorescent protein |
| 218012 | pMBS-854 | mVenus fluorescent protein |
| 218013 | pMBS-855 | mCherry fluorescent protein |
| 218014 | pMBS-856 | sfGFP fluorescent protein |
| 218015 | pMBS-872 | NanoLuc luminescence reporter |
| 218016 | pMBS-860 | mVenus fluorescent protein |
| 218017 | pMBS-861 | mCerulean fluorescent protein |
| 218018 | pMBS-864 | GST-tag fused to TEV Protease cleavage site for protein purification and cleavage |
| 218019 | pMBS-871 | 8xHis-1xHA-tag for protein detection and purification |
| 218020 | pMBS-1022 | 8xHis-SP20-3xHA-tag for protein detection and purification |
| 218021 | pMBS-869 | ER retention signal (DGDL) for protein targeting to ER |
| 218199 | pCM0-101 | Multi-Stop for adding 3 stops for each frame |
| 218201 | pCM0-099 | Strep-8xHis-tag for protein detection and purification |
| 218202 | pCM0-100 | 3xHA epitope tag for protein detection |
| 218203 | pMBS-723 | 1xHA-8xHis-tag for protein detection and purification |
| 218204 | pCM0-054 | SV40 NLS for protein targeting to nucleus |