Visualizing chaperone-mediated multistep assembly of the human 20S proteasome.
Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA
Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214137 | pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4 | Insect cell expression of the human 20SCP proteasome assembly chaperones |
| 214138 | pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7 | Insect cell expression of human 20S CP alpha-subunits |
| 214139 | pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII | Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2 |
| 214140 | pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7 | Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7 |
| 217893 | pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4 | Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4) |
| 217894 | pACEBac1-PSMA5-PSMA6-PSMA7 | Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7) |
| 217895 | pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4 | Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) |
| 217896 | pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII | Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag |
| 217897 | pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII | Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag |
| 217898 | pACEBac1-PSMB5-PSMB6-PSMB7 | Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) |