Visualizing chaperone-mediated multistep assembly of the human 20S proteasome.

Adolf F, Du J, Goodall EA, Walsh RM Jr, Rawson S, von Gronau S, Harper JW, Hanna J, Schulman BA
Nat Struct Mol Biol. 2024 Apr 10. doi: 10.1038/s41594-024-01268-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
214137	pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4	Insect cell expression of the human 20SCP proteasome assembly chaperones
214138	pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7	Insect cell expression of human 20S CP alpha-subunits
214139	pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII	Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
214140	pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7	Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
217893	pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4	Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)
217894	pACEBac1-PSMA5-PSMA6-PSMA7	Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)
217895	pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4	Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4)
217897	pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII	Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
217898	pACEBac1-PSMB5-PSMB6-PSMB7	Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)