Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes.
Yin JA, Frick L, Scheidmann MC, Liu T, Trevisan C, Dhingra A, Spinelli A, Wu Y, Yao L, Vena DL, Knapp B, Guo J, De Cecco E, Ging K, Armani A, Oakeley EJ, Nigsch F, Jenzer J, Haegele J, Pikusa M, Tager J, Rodriguez-Nieto S, Bouris V, Ribeiro R, Baroni F, Bedi MS, Berry S, Losa M, Hornemann S, Kampmann M, Pelkmans L, Hoepfner D, Heutink P, Aguzzi A
Nat Biomed Eng. 2024 Dec 4. doi: 10.1038/s41551-024-01278-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217778 | pYJA5 | The empty vector for quadruple sgRNA cloning |
| 217779 | hNTa1-qgRNA-pYJA5 | Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning |
| 217780 | hNTa2-qgRNA-pYJA5 | Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning |
| 217781 | hNTo1-qgRNA-pYJA5 | Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning |
| 217782 | hNTo2-qgRNA-pYJA5 | Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning |
| 217783 | CRISPRoff-mScarletI | Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing |