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Development of Genetically Encoded Fluorescent KSR1-Based Probes to Track Ceramides during Phagocytosis.

Girik V, van Ek L, Dentand Quadri I, Azam M, Cruz Cobo M, Mandavit M, Riezman I, Riezman H, Gavin AC, Nunes-Hasler P
Int J Mol Sci. 2024 Mar 5;25(5):2996. doi: 10.3390/ijms25052996. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217753pKSR1-CA3-EGFP (C-KSR)Fluorescent reporter for ceramide (mammalian expression)
217754pKSR1-CA3-mRFP1 (C-KSR)Fluorescent reporter for ceramide (mammalian expression)
217755pEGFP-KSR1-CA3 (N-KSR)Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
217756p2X-KSR1-CA3-EGFP (2x-C-KSR)Fluorescent reporter for ceramide (putative, mammalian expression)
217757pKSR1-CA3-GS-EGFP (C-KSR-GS)Fluorescent reporter for ceramide (mammalian expression)
217758pKSR1-CA3-GS-mRFP1 (C-KSR-GS)Fluorescent reporter for ceramide (mammalian expression)
217759pPRKCZ-C1-EGFP (C-PRKCZ-C1)Fluorescent reporter for ceramide (putative, mammalian expression)
217760pEGFP-PRKCZ-C1 (N-PRKCZ-C1)Fluorescent reporter for ceramide (putative, mammalian expression)
217761pPRKCZ-C20-EGFP (C-PRKCZ-C20)Fluorescent reporter for ceramide (putative, mammalian expression)
217762pEGFP-PRKCZ-C20 (N-PRKCZ-C20)Fluorescent reporter for ceramide (putative, mammalian expression)
217763pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)Fluorescent reporter for ceramide (putative, mammalian expression)
217764pSET-EMD-EGFP (C-SET-EMD)Fluorescent reporter for ceramide (putative, mammalian expression)
217765pEGFP-SET-EMD (N-SET-EMD)Fluorescent reporter for ceramide (putative, mammalian expression)
217766pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)Fluorescent reporter for ceramide (for bacterial expression and purification)
217767pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
217768pSbi-pur-KSR1-CA3-EGFP (C-KSR)Fluorescent reporter for ceramide (for stable mammalian expression)
217769pSbi-pur-EGFP-KSR1-CA3 (N-KSR)Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
217770pNES-SET-EMD-EGFP (C-NES-SET-EMD)Fluorescent reporter for ceramide (putative, mammalian expression)

Antibodies from Article