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Cohesin-mediated DNA loop extrusion resolves sister chromatids in G2 phase.

Batty P, Langer CC, Takacs Z, Tang W, Blaukopf C, Peters JM, Gerlich DW
EMBO J. 2023 Aug 15;42(16):e113475. doi: 10.15252/embj.2023113475. Epub 2023 Jun 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
217658RepairTemplate_Smc4-mAID-HaloTAGRepair template to endogenously tag SMC4 with mAID-Halo Tag
217659AAVS1_Cas9-hGem_gRNA2expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
217662gRNA3_NIPBL_Cas9-hGem-for_N-terminal_taggingexpresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
217664RepairTemplate_NIPBL_mEGFP_mAIDRepair template to endogenously tag NIPBL with mEGFP-mAID Tag
217665RepairTemplate_AAVS1-OsTIR1-F74G-SNAPRepair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus
217666Hygro-P2A-HA-FKBP-WAPLRepair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP
217667sgRNA1_Cas9-hGem_Smc4expresses Cas9-hGem and guideRNA for tagging of SMC4

Antibodies from Article