Restricting lysine normalizes toxic catabolites associated with ALDH7A1 deficiency in cells and mice.
Johal AS, Al-Shekaili HH, Abedrabbo M, Kehinde AZ, Towriss M, Koe JC, Hewton KG, Thomson SB, Ciernia AV, Leavitt B, Parker SJ
Cell Rep. 2024 Dec 24;43(12):115069. doi: 10.1016/j.celrep.2024.115069. Epub 2024 Dec 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217653 | pLentiCMV_HyPer7RgDAAO | Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane |
| 217746 | pDONR223_HyPer7RgDAAO | Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane. |
| 218273 | pLentiCRISPRv2_sgALDH7A1 | CRISPR KO plasmid for ALDH7A1 in human cell lines |
| 218346 | pLentiCRISPRRv2_sgTom | CRISPR KO plasmid for tdTomato in human cell lines |