Restricting lysine normalizes toxic catabolites associated with ALDH7A1 deficiency in cells and mice

Johal AS, Al-Shekaili HH, Abedrabbo M, Kehinde AZ, Towriss M, Koe JC, Hewton KG, Thomson SB, Ciernia AV, Leavitt B, Parker SJ
Cell Reports, Volume 43, Issue 12, 115069 Article

Plasmids from Article

ID	Plasmid	Purpose
217653	pLentiCMV_HyPer7RgDAAO	Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
217746	pDONR223_HyPer7RgDAAO	Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
218273	pLentiCRISPRv2_sgALDH7A1	CRISPR KO plasmid for ALDH7A1 in human cell lines
218346	pLentiCRISPRRv2_sgTom	CRISPR KO plasmid for tdTomato in human cell lines