Cell-free assays reveal that the HIV-1 capsid protects reverse transcripts from cGAS immune sensing.

Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI, Johnson JS
PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 10.1371/journal.ppat.1012206. PubMed Article

Plasmids from Article

Showing 1 to 10 of 13 entries

Show 102550100All entries

Search table:

• Previous
• 1
• 2
• Next

ID	Plasmid	Purpose
217449	pCA528 huPQBP1	Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector
217448	LCV2 PQBP1 KO2	Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1
217447	LCV2 PQBP1 KO1	Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1
217446	LCV2 IRF3 KO	Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3
217445	LCV2 STING KO	Lentiviral vector expressing Cas9 and an sgRNA targeting human STING
217444	LCV2 cGAS KO	Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS
217443	LCV2 control	Lentiviral vector expressing Cas9 without a targeting sgRNA
217442	SG3ΔENV CA Q67H/N74D	Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).
217441	HIV-1-GFP ΔEnv CA Q67H/N74D	HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).
217440	HIV-1-GFP ΔEnv CA M66I	HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).