Cell-free assays reveal that the HIV-1 capsid protects reverse transcripts from cGAS immune sensing.
Scott TM, Arnold LM, Powers JA, McCann DA, Rowe AB, Christensen DE, Pereira MJ, Zhou W, Torrez RM, Iwasa JH, Kranzusch PJ, Sundquist WI, Johnson JS
PLoS Pathog. 2025 Jan 28;21(1):e1012206. doi: 10.1371/journal.ppat.1012206.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217437 | HIV-1-GFP ΔEnv | HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu. |
| 217438 | HIV-1-GFP ΔEnv CA E45A | HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA). |
| 217439 | HIV-1-GFP ΔEnv CA Q63/67A | HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA). |
| 217440 | HIV-1-GFP ΔEnv CA M66I | HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA). |
| 217441 | HIV-1-GFP ΔEnv CA Q67H/N74D | HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA). |
| 217442 | SG3ΔENV CA Q67H/N74D | Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D). |
| 217443 | LCV2 control | Lentiviral vector expressing Cas9 without a targeting sgRNA |
| 217444 | LCV2 cGAS KO | Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS |
| 217445 | LCV2 STING KO | Lentiviral vector expressing Cas9 and an sgRNA targeting human STING |
| 217446 | LCV2 IRF3 KO | Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3 |
| 217447 | LCV2 PQBP1 KO1 | Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1 |
| 217448 | LCV2 PQBP1 KO2 | Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1 |
| 217449 | pCA528 huPQBP1 | Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector |