Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations.
Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA
Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02224-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217330 | pCH45 (multiAsCas12a-KRAB lenti) | Lentiviral expression of multiAsCas12a-KRAB |
| 217331 | pCH4 (denAsCas12a-KRAB lenti) | Lentiviral expression of denAsCas12a-KRAB |
| 217332 | pCH61 (multiAsCas12a-KRAB piggyBac) | piggyBac expression of multiAsCas12a-KRAB |
| 217333 | pCH62 (denAsCas12a-KRAB piggyBac) | piggyBac expression of denAsCas12a-KRAB |
| 217334 | pCH68 (dAsCas12a-3xKRAB piggyBac) | piggyBac expression of dAsCas12a-3xKRAB |
| 217335 | pNS49 (enAsCas12a-KRAB piggyBac) | piggyBac expresion of enAsCas12a-KRAB |
| 217336 | pNS35 (multiAsCas12a piggyBac) | piggyBac expression of multiAsCas12a |
| 217337 | pCH67 (crRNA expression vector) | CROP-seq crRNA expression vector with GFP and puro marker |
| 217338 | pCH49 (crRNA expression vector) | CROP-seq crRNA expression vector with GFP and neo marker |
| 217339 | gCH128 (non-targeting crRNA) | Non-targeting crRNA |
| 217340 | gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1) | crRNA array targeting CD81, B2M, KIT, CD55 |
| 217341 | gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1) | crRNA array targeting CD81, B2M, KIT, CD55 |
| 217342 | gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1) | crRNA array targeting CD81, B2M, KIT, CD55 |
| 217343 | gCH27 (crCD55-4) | crRNA targeting CD55 |
| 217344 | gCH29 (crCD81-1) | crRNA targeting CD81 |
| 217345 | gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1) | crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81 |