Protein engineering enables a soakable crystal form of human CDK7 primed for high-throughput crystallography and structure-based drug design.
Mukherjee M, Day PJ, Laverty D, Bueren-Calabuig JA, Woodhead AJ, Griffiths-Jones C, Hiscock S, East C, Boyd S, O'Reilly M
Structure. 2024 Jun 9:S0969-2126(24)00188-6. doi: 10.1016/j.str.2024.05.011.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217012 | pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346 | Expression of CDK7 in insect cells |
| 217013 | pFastBac dual_Human full length CDK7(W132R, S164D, T170E) | Expression of CDK7 in insect cells |
| 217014 | pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) | Expression of CDK2-7 chimera in E.coli |