In vivo proximity proteomics uncovers palmdelphin (PALMD) as a Z-disc-associated mitigator of isoproterenol-induced cardiac injury.

Guo CT, Jardin BD, Lin JS, Ambroise RL, Wang Z, Yang LZ, Mazumdar N, Lu FJ, Ma Q, Cao YP, Liu CZ, Li KL, Liu XJ, Lan F, Zhao MM, Xiao H, Dong ED, Pu WT, Guo YX
Acta Pharmacol Sin. 2024 Jul 23. doi: 10.1038/s41401-024-01348-y. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
216835	pAAV-cTNT-NEXN-GFP	cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Nexn and GFP tag
216834	pCMV-3*flag-NEXN	expressing Falg tag and the coding sequence of mouse Nexn
216833	pCMV-3*HA-Palmd	expressing HA tag and the coding sequence of mouse Palmd
216832	pAAV-cTNT-GFP-Palmd	cardiomyocytes-specific plasmid, expressing Gfp and the coding sequence of mouse Palmd
216831	pENAAV-cTNT-actn2-BioID2-HA	cardiomyocytes-specific plasmid, expressing the coding sequence of mouse Actn2, biotin ligase BioID2, and a HA tag.
216830	AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS	cardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.