Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system.
Modaffari D, Finlayson A, Miao Y, Wallace EWJ, Sawin KE
Wellcome Open Res. 2024 Oct 17;9:602. doi: 10.12688/wellcomeopenres.23086.1. eCollection 2024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216808 | pDM026 | Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker. |
| 216809 | pDM028 | Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker. |
| 216810 | pDM030 | Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker. |
| 216811 | pDM068 | Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker. |
| 216812 | pDM042 | Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for C-terminal tagging. |
| 216813 | pDM043 | Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging. |
| 216814 | pDM052 | Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for C-terminal tagging. |
| 216815 | pDM053 | Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging. |
| 216816 | pDM070 | Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging. |
| 216817 | pDM071 | Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging. |
| 216818 | pDM098 | Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging. |
| 216819 | pDM097 | Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging. |
| 216820 | pDM067 | Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for C-terminal tagging. |
| 216821 | pDM069 | Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging. |