Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems.
Zhong BL, Elliot JM, Wang P, Li H, Hall RN, Wang B, Prakash M, Dunn AR
ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216714 | T7-LacO-Halo-PILATeS-RGD (700pM) | Expresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteria |
| 216715 | T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert) | Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria |
| 216716 | T7-LacO-Halo-PILATeS-RGD (200nM) | Expresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteria |
| 216717 | T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert) | Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria |
| 216761 | mCh-LgBiT-2A-EGFP-Vin-PILATeS | PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase |
| 216763 | mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT | PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase |
| 216779 | T7-LacO-LgBiT | Expresses LgBiT in bacteria |