A dual reporter system for intracellular and extracellular amino acid sensing in budding yeast.
Paukstyte J, Tena EC, Saarikangas J
Mol Biol Cell. 2025 Apr 2:mbcE24040162. doi: 10.1091/mbc.E24-04-0162.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216512 | pCfB2188-PrARG8-GFP::URA3 | Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast |
| 216513 | pCfB2197-PrGNP1-mK2-NatMX | Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast |