Generation of human alveolar epithelial type I cells from pluripotent stem cells.
Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, Kotton DN
Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 10.1016/j.stem.2024.03.017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216470 | p2701-AGER-tdTomato | donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site |
| 216471 | p2702-AGER-gRNA1 | to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site |
| 216472 | p2703-AGER-gRNA2 | to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site |
| 216473 | p2704-AGER-gRNA3 | to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site |
| 216474 | p2705-AGER-eGFP | donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site |
| 216475 | p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP | lentiviral dual expression of YAP5SA and eGFP |
| 216476 | p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP | lentiviral dual expression of dsRed and tagBFP |
| 216477 | p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP | lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision |
| 216478 | p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP | lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision |
| 216479 | p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP | lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision |
| 216480 | p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP | lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision |