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Far-red and sensitive sensor for monitoring real time H2O2 dynamics with subcellular resolution and in multi-parametric imaging applications

Lee J.D., Nguyen A., Jin Z.R., Moghadasi A., Gibbs C.E., Wait S.J., Evitts K.M., Asencio A., Bremner S.B., Zuniga S., Chavan V., Williams A., Smith N., Regnier M., Young J.E., Mack D., Nance E., Boyle P.M., Berndt A.
bioRxiv 2024.02.06.579232 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216412pC1_oROS-HT_LF(C199S)Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.
216413pC1_oROS-HTExpresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.
216414AAV2_CAG_oROS-HT_WPREEncodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors
216415AAV2_CAG_oROS-HT(C199S)_WPREEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors
216416pDisplay-oROS-HTTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.
216417pC1-oROS-HT-CaaXTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.
216418pC1-dmito-oROS-HTTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.
216419pC1-IMS-oROS-HTTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.
216420pC1-lifeact-oROS-HTTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

Antibodies from Article