The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling
Bruguera ES, Mahoney JP, Weis WI
bioRxiv 2024.02.03.578714
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216379 | mFzd4CRDL(42-203)-6xHis_pAcGP67a | Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells |
| 216380 | FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393 | Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10) |
| 216381 | hTspan12(1-305)-1D4_pTT5 | Mammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tag |
| 216382 | hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393 | Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11 |
| 216383 | MBP-3C-hTspan12LEL(118-218)_pAcGP67a | Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP |
| 216384 | MBP-3C-hNorrin(33-133)-1D4_pAcGP67a | Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) |
| 216385 | MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a | Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A) |