PI(4,5)P(2)-stimulated positive feedback drives the recruitment of Dishevelled to Frizzled in Wnt-beta-catenin signaling.
Mahoney JP, Bruguera ES, Vasishtha M, Killingsworth LB, Kyaw S, Weis WI
Sci Signal. 2022 Aug 23;15(748):eabo2820. doi: 10.1126/scisignal.abo2820. Epub 2022 Aug 23.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216378 | FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1 | Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression) |
| 216386 | 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet | Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353) |
| 216387 | 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet | Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510) |