mRNA trans-splicing dual AAV vectors for (epi)genome editing and gene therapy.
Riedmayr LM, Hinrichsmeyer KS, Thalhammer SB, Mittas DM, Karguth N, Otify DY, Bohm S, Weber VJ, Bartoschek MD, Splith V, Brummer M, Ferreira R, Boon N, Wogenstein GM, Grimm C, Wijnholds J, Mehlfeld V, Michalakis S, Fenske S, Biel M, Becirovic E
Nat Commun. 2023 Oct 18;14(1):6578. doi: 10.1038/s41467-023-42386-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216318 | pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA | Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system). |
| 216319 | pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA | Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system). |
| 216320 | pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA | Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system). |
| 216321 | pAAV-CMV-BDLacZ-SAS620-3'Luciferase (split CAGGT)-SV40pA | Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system). |
| 216322 | pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA | Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system). |
| 216323 | pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA | Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system). |