SpyMask enables combinatorial assembly of bispecific binders.
Driscoll CL, Keeble AH, Howarth MR
Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216280 | pET28a-SnoopTag-AffiHER2-SpyTag | Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression |
| 216281 | pET28a-SnoopTag-SpyTag-(AffiHER2)x3 | Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher |
| 216282 | pET28a-SUMO-SpyTag003 | Expresses SUMO with a C-terminal SpyTag003 in bacterial cells |
| 216283 | pDEST14-SpyCatcher003-TEVs-SpyTag003DA | Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity. |
| 216284 | pDEST14-DoubleCatcher | Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders |
| 216285 | pDEST14-DoubleCatcher-H-Lock | Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker. |
| 216286 | pDEST14-DoubleCatcher-⍺-Lock | Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond. |
| 216287 | pDEST14-DoubleCatcher-β-Lock | Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond. |
| 216288 | pDEST14-DoubleCatcher-γ-Lock | Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond. |
| 216289 | pDEST14-DoubleCatcher-δ-Lock | Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond. |
| 216290 | pDEST14-DoubleCatcher-ε-Lock | Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond. |
| 216292 | pcDNA3.1-Tras heavy chain-GSG-SpyTag003 | Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab |
| 216293 | pcDNA3.1-Tras heavy chain-SpyTag003 | Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab |
| 216295 | pET28-SpyCatcher002-MBP | Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells |
| 216296 | pcDNA3.1-39S Heavy chain-SpyTag003 | Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab |
| 216297 | pcDNA3.1-39S Light chain | Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab |
| 216303 | pcDNA3.1-MF3958 Heavy chain-SpyTag003 | Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab |
| 216304 | pcDNA3.1-MF3958 Light chain | Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab |
| 216307 | pcDNA3.1-H2-18 Heavy chain-SpyTag003 | Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab |
| 216308 | pcDNA3.1-H2-18 Light chain | Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab |
| 216309 | pcDNA3.1-Pert Heavy chain-SpyTag003 | Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab) |
| 216312 | pET28a-nanoHER2-SpyTag003 | Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody. |