A platform for predicting mechanism of action based on bacterial transcriptional responses identifies an unusual DNA gyrase inhibitor.
French S, Guo ABY, Ellis MJ, Deisinger JP, Johnson JW, Rachwalski K, Piquette ZA, Lluka T, Zary M, Gamage S, Magolan J, Brown ED
Cell Rep. 2024 Apr 4;43(4):114053. doi: 10.1016/j.celrep.2024.114053.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216229 | pET151-GyrA | S. aureus USA300 GyrA Codon-optimized for E. coli |
| 216230 | pET151-GyrA_F164R238 | S. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coli |
| 216233 | pET151-GyrB | S. aureus USA300 GyrB Codon-optimized for E. coli |
| 216234 | pET151-GyrB_A467 | S. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coli |
| 216235 | pET151-GyrB_T522 | S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli |