CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens
Walton RT, Qin Y, Blainey PC
bioRxiv 2024.03.17.585235
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Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
216217 | CROPseq-multi-Puro (pRW1441) | Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance |
216218 | CROPseq-multi-Zeo (pRW1445) | Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance |
216219 | CROPseq-multi-EBFP2-NLS (pRW1447) | Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression |
225752 | CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484) | Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection |
225753 | CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500) | Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection |
225754 | CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506) | Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection |
225755 | CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509) | Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection |
225756 | CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512) | Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection |