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CROPseq-multi: a versatile solution for multiplexed perturbation and decoding in pooled CRISPR screens

Walton RT, Qin Y, Blainey PC
bioRxiv 2024.03.17.585235 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216217CROPseq-multi-Puro (pRW1441)Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance
216218CROPseq-multi-Zeo (pRW1445)Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistance
216219CROPseq-multi-EBFP2-NLS (pRW1447)Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression
225752CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
225753CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection
225754CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection
225755CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection
225756CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Antibodies from Article