POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology.
Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216426 | pPOM001_P1_ConL6 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216427 | pPOM002_P1_ConL7 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216428 | pPOM003_P1_ConL8 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216429 | pPOM004_P1_ConL9 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216430 | pPOM005_P1_ConL10 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216431 | pPOM006_P1_ConL11 | Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector. |
| 216432 | pPOM007_P5_ConR6 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216433 | pPOM008_P5_ConR7 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216434 | pPOM009_P5_ConR8 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216435 | pPOM010_P5_ConR9 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216436 | pPOM011_P5_ConR10 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216437 | pPOM012_P5_ConR11 | Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector. |
| 216438 | pPOM013_P2_pENO101 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216439 | pPOM014_P2_pGPM1 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216440 | pPOM015_P2_pADH1 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216441 | pPOM016_P2_pRPL2501 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216442 | pPOM017_P2_pTIF51 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216443 | pPOM018_P2_pRPL701 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216444 | pPOM019_P2_pRPL2102 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216445 | pPOM020_P2_pTRS1 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216446 | pPOM021_P2_pTUB1 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216447 | pPOM022_P2_pRPS1002 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216448 | pPOM023_P2_pAPL4 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216449 | pPOM024_P2_pMPA1 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216450 | pPOM025_P2_pTUP11 | Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216451 | pPOM026_P2_pNMT1 | Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar. |
| 216452 | pPOM027_P4_tSynthGuo | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216453 | pPOM028_P4_tSynth3 | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216454 | pPOM029_P4_tSynth25 | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216455 | pPOM030_P4_tSynth27 | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216456 | pPOM031_P4_tSynth29 | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216457 | pPOM032_P4_tSynth30 | Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar. |
| 216458 | pPOM033_P7_3"His5 | 3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar. |
| 216459 | pPOM034_P7_3"Lys3 | 3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar. |
| 216461 | pPOM040_P8b_5'Ura4 | 5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar. |
| 216463 | pPOM038_P8b_5'Lys3 | 5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar. |
| 216465 | pPOM036_P7_3"Ura4 | 3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar. |
| 216466 | pPOM041_P15678_SingleGeneBackbone | Empty integration vector for genomic integration of 1 transcriptional unit into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out. |
| 216467 | pPOM042_P15678_MultiGeneBackbone | Empty integration vector for genomic integration for multigene into S. pombe. Targets Ura4 locus. Part type 15678 following the YeastToolkit MoClo grammar. Contains GFP drop out. |