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Improved validation of protein interactions using bicistronic BiFC (Bi2FC).

Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 10.1007/s12298-024-01477-y. Epub 2024 Jul 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216136pBi2FC-N-MPK5-P2A:mCherryControls for Bicistronic BiFC
216137pBi2FC-N-MPK6-P2A:mCherryControls for Bicistronic BiFC
216138pBi2FC-N-MPK8-P2A:mCherryControls for Bicistronic BiFC
216139pBi2FC-N-MPK12-P2A:mCherryControls for Bicistronic BiFC
216140pBi2FC-C-GRF4-P2A:mTagBFP2Controls for Bicistronic BiFC
216141pBi2FC-C-MGGAGM-P2A:mTagBFP2Vector control for Bicistronic BiFC
216142pBi2FC-N-MGGAGM-P2A:mCherryVector control for Bicistronic BiFC
216143pBi2FC-C-GW-P2A:mTagBFP2Bicistronic BiFC vector (Gateway compatible)
216144pBi2FC-N-GW-P2A:mCherryBicistronic BiFC vector (Gateway compatible)
216145pCENTR_MGGAGMEntry Vector
216146pCONR221Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

Antibodies from Article