Pooled multicolour tagging for visualizing subcellular protein dynamics.
Reicher A, Reinis J, Ciobanu M, Ruzicka P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S
Nat Cell Biol. 2024 May;26(5):745-756. doi: 10.1038/s41556-024-01407-w. Epub 2024 Apr 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216123 | CROPseq-Guide-BSD | Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter. |
| 216124 | Intron-Tagging-EGFP-Frame0-Parental-Minicircle | Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes. |
| 216125 | Intron-Tagging-mScarlet-Frame1-Parental-Minicircle | Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes. |
| 216126 | lenti-mTagBFP2 | Lentiviral vector for expressing mTagBFP2. |
| 216127 | lenti-ER-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum. |
| 216128 | lenti-NLS-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the nucleus |
| 216129 | lenti-NES-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm |
| 216130 | lenti-mito-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to mitochondria. |
| 216131 | lenti-NLS-miRFP670nano-miRFP670 | Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus. |
| 216132 | lenti-mAmetrine-CAAX | Lentiviral vector for expressing mAmetrine localizing to the plasma membrane. |
| 216133 | Human Genome-wide Intron Tagging Library, Frame 0 | Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. |
| 216134 | Human Genome-wide Intron Tagging Library, Frame 1 | Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. |