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Structure-guided engineering of a fast genetically encoded sensor for real-time H2O2 monitoring

Lee J.D., Won. W., Kimball K., Wang Y., Yeboah F., Evitts K.M., Neiswanger C., Schattauer S., Rappleye M., Bremner S.B., Chun C., Smith N., Mack D.L., Young J.E., Lee C.J., Chavkin C., Berndt A.
bioRxiv 2024.01.31.578117 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216111pC3.1_CMV_oROS-GExpresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.
216112pC3.1_CMV_oROS-G_LF(C199S)Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.
216113pC3.1_CAG_oROS-GrExpresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.
216114pC3.1_CAG_oROS-Gr_LF(C199S)Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.
216115AAV2_CAG_oROS-GEncodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.
216116AAV2_CAG_oROS-G_LF(C199S)Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Antibodies from Article