Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate.
Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M
Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 10.1016/j.molcel.2023.12.007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216161 | pLV GG hUbV-EGFP | Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP |
| 216170 | FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd | Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment |
| 216171 | FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd | Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment |
| 216172 | FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd | Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment |
| 216173 | LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2 | Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment |
| 216531 | pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry) | Targeting vector for CRISPRd generation |