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A robust model for quantitative prediction of the silencing efficacy of wild-type and A-to-I edited miRNAs.

Tian S, Terai G, Kobayashi Y, Kimura Y, Abe H, Asai K, Ui-Tei K
RNA Biol. 2020 Feb;17(2):264-280. doi: 10.1080/15476286.2019.1678364. Epub 2019 Nov 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
215875psiCHECK-1-miR-376a1(5p)-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215876psiCHECK-1-miR-376a1(3p)-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215877psiCHECK-1-miR-589(5p)-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215878psiCHECK-1-miR-589(3p)-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215879psiCHECK-1-miR-376a2(3p)-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215880psiCHECK-1-miR-99a-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215881psiCHECK-1-miR-455-WTThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215882psiCHECK-1-miR-376a1(5p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215883psiCHECK-1-miR-376a1(3p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215884psiCHECK-1-miR-589(5p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215885psiCHECK-1-miR-589(3p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215886psiCHECK-1-miR-22(3p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215887psiCHECK-1-miR-376a2(3p)-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.
215888psiCHECK-1-miR-99a-I-typeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Antibodies from Article