A selection of Golden Gate vectors to simplify recombinant protein production in Escherichia coli
Fairhead M, Koekemoer L, Williams E, von Delft F
bioRxiv 2024.02.13.579886
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215789 | pNIC-NHSTIIT-GG | Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage |
| 215790 | pNIC-OmpA-NHSTIIT-GG | Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage |
| 215791 | pNIC-SufI-NHSTIIT-GG | Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage |
| 215792 | pNIC-TolB-NHSTIIT-GG | Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage |
| 215793 | pNIC-PelB-NH10T-AVITAG-GG | Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage |
| 215794 | pNIC-MBP-TEV-GG | Cloning of target gene with N terminal MBP tag and TEV cleavage |
| 215795 | pNIC-GST-TEV-GG | Cloning of target gene with N terminal GST tag and TEV cleavage |
| 215796 | pNIC-HIS6-THRX-TEV-GG | Cloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavage |
| 215797 | pNIC-HIS10-ZBASIC-TEV-GG | Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage |
| 215798 | pNIC-HIS6-NUSA-TEV-GG | Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage |
| 215799 | pNIC-HIS6-SUMO1-GG | Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage |
| 215800 | pNIC-HIS6-MBP-TEV-GG | Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage |
| 215801 | pNIC-HIS6-GST-TEV-GG | Cloning of target gene with N terminal His6 GST tag and TEV cleavage |
| 215802 | pNIC-NHSTIIT-AVITAG-GG | Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG |
| 215803 | pNIC-CT-sfGFP-HN-GG | Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag |
| 215804 | pNIC-CTH10STII-GG | Cloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag |
| 215805 | pNIC- AVITAG-CTH10STII- -GG | Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag |
| 215806 | pNIC-NH10-GG | Cloning of target gene with N terminal non cleavable His10 tag |
| 215807 | pNIC-CH10-GG | Cloning of target gene with C terminal non cleavable His10 tag |
| 215808 | pNIC-HIS6-SUMO1-AVITAG-GG | Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag |
| 215809 | pNIC-HIS6-GST-TEV-AVITAG-GG | Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag |
| 215810 | pNIC-NoTag-GG | Cloning of target gene with no tags |